Fig 1: RSK2 co-localizes with FLNaU-373 MG cells were grown on coverslips coated with 10 µg/ml fibronectin. Cells were serum starved overnight and then stimulated for 5 or 30 min with 10 ng/ml EGF. Cells were fixed and stained for RSK2 and FLNa using specific primary antibodies. Immunostaining was visualized with confocal microscopy using a 63x objective. Scale bars shown represent 30 µm (5 µm in the zoom pictures).